Am using a 160mM HEPES buffer and diluting serum by 10x and 200x. Found this [ ] works with blood.
pH of buffer is 7.4 and serum was kept at either 4C or RT.
Disperant T was altered from 4C to 37C, allowing for a 2 min equilibration time.
Conducivity lowered with decreasing Temp: 37 (4.74) to 4 (1.94)
Used a voltage of 50V.
4C: -4 AVG ZP
25C: -7.5
37C: -10
For every measure I got "count rate varies- sample not stable clean"
Can anyone point out some literature sources or provide technical support?
Most probably you are heating or electrolyzing your sample.
Which version of the instrument? What units are the conductivity values? What kind of sample cell are you using?
I would expect both zeta and conductivity to drop with decreasing temperature (as observed).
If you can, please post a .dta file.
This is most likely that your sample is being aggregated. Another way can be to dilute the sample and run the measurement. Good luck.
Daniel Villarreal When you say serum, does that imply that you have multiple protein species present? If so, zeta potentials reported by the instrument will be of very little use because it measures an average value (if you have a Zetasize Nano or newer running in monomodal mode) or utter gibberish if running in the mode that gives you a distribution.
Please post an example .dta file so I can look at the various diagnostic information.
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