What conditions and anti-FLAG antibody are proven to work in tissue sections of mouse?

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fatal error

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Béatrice Marianne Ewalds-Kvist

Dear Joseph,

you can always check with the authors to these papers here on RG:

Article Expression and Intracellular Traffic of the Newly Synthesize...

Article Immunohistochemical Detection of FLAG-Tagged Endogenous Prot...

Joseph Miano

Thanks. The second one we have and it seems not so compelling. Will check other one.

Cheers

Joe

Lalhaba Oinam

Dear Joseph, I am having a similar trouble if you find anything new please do update.

Regards,

Lal

we see nuclear staining in non-FLAG mouse tissues, Very disconcerting when our tagged gene encodes a transcription factor! We are going to preabsorb the FLAG antibody with a peptide and see if we can show that the non-specific staining remains. Meanwhile, we have recently had better luck with an HA tag.