What concentration of monodensylchadaverine should be used in neuroblastoma cell line(SH-SY5Y)?

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Can anyone help to convert ODK XLS into XML?

We want to conduct a verbal autopsy using WHO adult VA updated tools in ODK. The current version of WHO adult verbal autopsy tools is v1.5.3. I have successfully converted VA ODK-XLS v1.4 into XML...

03 February 2021 844 2 View

Hello every one! How can i design the primer for mature microRNA for RT-PCR quantification?

I am going to validate some microRNA genes in two developmental stages. But i found the method for miRNA primer designing is totally change compering to mRNA and LncRNA primers designing. Please...

03 December 2020 4,555 6 View

Measuring NO NO2 and Nitrous acid?

Hi can any one out there please help me, I want to measure NOx release from one of our product in gas phase and in liquid phase. how can I measure and quantify it? Please help us if any one can. I...

19 October 2020 6,365 1 View

Path tracing of vehicle in a video using Python ?

I want to trace vehicles using Python path as shown in the video https://www.youtube.com/watch?v=BP1OMyYuyy4 I have detected bounding box and have centroid location for every car for each frame....

13 March 2020 1,287 3 View

How to calculate MOSFET losses in an inverter topology at different power levels?

I want to simulate an inverter topology using PSIM thermal module to calculate MOSFET switching and conduction losses at different power levels. How I can adjust different power levels in...

24 February 2020 7,122 2 View

Where can I get these parameters to simulate DSSC solar cell in Silvaco Atlas?

I want to simulate the DSSC solar cells using Silvaco Atlas .Required Parameters: Non-radiative singlet exciton decay rate (s-1)-KNRS.EXCITON Exciton diffusion length (nm)-LDS.EXCITON Number of...

01 December 2019 7,792 4 View

I have the experimental data available for solar dryer, ( moisture ratio vs time). How to calculate predicted moisture ratio using regression method?

How to determine coefficients (a,c,k and n) of different drying models(Newton, Lewis, Modified Page models etc.) using multiple regression method?

15 October 2019 7,592 4 View

Which approach is better and cost effective for detecting mutations in atp1a3 gene? Using mRNA and cDNA or Whole Exome Sequencing?

Hi. I am planning a screening study for apt1a3 gene in a family with clinical manifestations. The protein is about 1013 amino acids long, so its coding part is 3042 nucleotides. Numerous mutations...

06 October 2019 7,655 6 View

Any mechanism to find out the citation index and h-index of a department as a whole?

We can have information about the citation and h-index of an individual. If I wish to find out these parameters for a department / a particular Lab, where I will get such information? Any...

19 September 2019 1,085 3 View

Oxygen scavenging from gas mixtures when analyzed on GC?

How to scavenge oxygen from a gas mixtures as oxygen and argon are detected indistinguishably by TCD detector? Any other method except pyrogallol?

03 July 2019 6,889 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Yubin Li

would you use MDC for autophagy detecting? If so, you can incubate cells with 0.05mM MDC for 10 min.

Ibrar Siddique

i did it, even on 0.025mM concentration cells start leaving the surface of the cover slip which was coated with poly L-lysine

Attila Lehotzky

Try to cover something else, a charged similar compound, like mdc disturbs the cells' attachement.

can you suggest me the compound which can be used in place of poly L-lysine