Ibrar Siddique

8 Questions 8 Answers 0 Followers

Questions related from Ibrar Siddique

Hi Everyone, I am facing the problem in the co-localisation study of the two proteins in the cell using the Confocal microscopy ?

Actually, i am trying the co-localize two proteins one of them is ER localize. The signals appear in the whole cell and difficult to localize. I am thinking a way to get rid of the cytoplasm from...

14 December 2017 2,815 2 View

How can I avoid the granules like structure on the surface of the cells?

Hello Everyone,We are facing a problem in all cell lines running in our culture room. After two or three passages, the cells start showing the granules like structure on its surface. They...

13 April 2016 9,866 3 View

I am working on Alzheimer disease and I plan to show the Abeta42 level in the mouse brain homogenate by western blotting, should I proceed?

For this western blot, i am running the tricine-sds-page. I run the gel until the 10KD band of the ladder has traveled half of the gel. But, when i go for the commassie or silver staining, i dont...

01 July 2015 525 10 View

Can you help me on Silver staining of brain sections for amyloid depositions?

I am working on the Alzheimer's disease and I have done ThS staining and I will be proceeding for the silver staining. Can anyone give me the protocol for the silver staining of a brain section?

06 January 2015 1,788 4 View

My fusion protein has a MW of about 46KD. The band appears at about 40 KD, in purified form and in the lysate too. Can any one tell me the reason?

I have cloned a gene in pgex-4t1 vector, which is gst tag. My protein is about 20KD. While band appear at about 40KD. I have confirmed the clone by sequencing and double digestion. And also...

20 November 2014 5,051 6 View

How can I measure cholesterol level in my cell lysate?

I want to see the change in the cholesterol level in my cells after the treatment. I am using the SH-SY5Y cell line.

10 November 2014 6,379 11 View

What concentration of monodensylchadaverine should be used in neuroblastoma cell line(SH-SY5Y)?

I have tried conc of the dye from o.o25mM to the 0.1mM but the cell death starts on all conc

26 December 2013 9,751 4 View

No puncta appears after the transfection of eGFP-LC3, why?

I am working on autophagy, I have seen the downrwgulaion and upregulation of autophagy by LC3B using Western Blot. When I tried to show this using eGFP-LC3 transfection, whole cell fluoresce, and...

01 January 1970 3,301 4 View