H2O2 is an assay used to measure the antioxidant activity of an extract. There are different concentrations of H2O2 available. However, literature doesn't clearly cite the exact concentration of H2O2 that needs to be used.
The concentrations in the millimolar range (e.g., 20 mM or 40 mM) are commonly employed in the H2O2 scavenging assay for plant extracts. The choice of H2O2 concentration may depend on the specific plant extract, the desired level of oxidative stress, and the method used to quantify the antioxidant activity.
The highest H2O2 scavenging activity was at 200 µg/ml concentration (84.47%) and the wavelength was 230nm
Hi Nethra,
H2O2 scavenging activity is calculated in percentage. For this, H2O2 solution (prepared in phosphate buffer (pH 7.4)) of 40 mM concentration is used. Please see research publication for details
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DOI 10.1007/s00709-014-0613-4
Schopfer P, Plachy C, Frahry G (2001) Release of reactive oxygen
intermediates (superoxide radicals, hydrogen peroxide, and hydroxyl
radicals) and peroxidase in germinating radish seeds controlled by
light, gibberellin, and abscisic acid. Plant Physiol 125:1591–1602
Vajanathappa J, Badami S (2009) Antiedematogenic and free radical
scavenging activity of swertiamarin isolated from Enicostemma
axillare. Planta Med 75:12–17
I was curious about opinions of scholars before to post my answer. First, the question is ill formulated. "H2O2 is an assay used to measure the antioxidant activity of an extract." It's not specified which assay. The question in the current form does not have any sense. The answers are very general and don't specify which assay as well.
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