Nethra P V

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Related to starch kinetics, amylase inhabition assay. No colour change was seen, what could be the possible reason?

I'm checking the inhibition of amylase enzyme through starch hydrolysis. I will explain the protocol here: Reagents: PBS buffer- 20 mM, 6.9 pH, with 0.1 mM Cacl2 and 6.7 mM Nacl 40 U amylase 50...

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What concentration of H2O2 is used for H2O2 scavenging activity?

H2O2 is an assay used to measure the antioxidant activity of an extract. There are different concentrations of H2O2 available. However, literature doesn't clearly cite the exact concentration of...

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What are the internal standards that can be used for the analysis of amino acids (Pre-Column derivatization by 6-Aminoquinoline) using HPLC?

I need to derivatize amino acids from a plant based beverage sample, what is the best derivatization method and the internal standard to be used while running HPLC?

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