Hello all,
I have an Agilent 1220 HPLC with DAD, and when I run analysis, I see something to the tune of 26 peaks in my chromatogram. My experiments are greatly dependent on the reliability of my results. I am working to remove delta 8 and 9 THC from hemp oil, using a novel method. I am using 11 cannabinoid standards for a curve. However, as mentioned above, I am seeing 26 peaks, and many fall within the relative time window of THC. I want to make sure I am getting results I can trust, since my scale up design for production is dependent on getting LOQ results. I am limited on the bench scale however many of the constraints will be resolved by my scale up design.
So my question lays within, how can I ensure that the quantitative results are being associated with the right compounds, since there seems to be many other peaks?
I speculate that since the oil was originally extracted using ethanol, that I am getting flavonoids in my mix. During distillation I imagine some lipids and carotenoids are making it into the matrix since I see a reddish/orange hue. Any feedback will be greatly appreciated.
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