What are the steps to make a hybrid analysis of inputs and outputs to calculate the embodied energy?

My colleagues and I are doing an article on the calculation of the grey energy from the implementation of renewable energy sources; such as solar, wind and pch, in Colombia. So, researching we...

21 January 2020 7,611 4 View

Has anyone had trouble running the testosterone ELISA kit from Cayman Chemicals?

I use Cyaman Chm ELISA kits (https://www.caymanchem.com/app/template/Product.vm/catalog/582701). The testosterone kit has shown to be unreliable. The results have not been reproducible. A large...

12 January 2015 486 4 View

Does anybody know why in the last step of MSP1D1 protein purification this protein precipitates?

05 October 2014 8,744 5 View

Which kind of LFS could I use to induce LTD in acute hippocampal slices?

I would like to know which kind of LFS (low frequency stimulation) I could consider using as a weak protocol to induce LTD in acute slices from 3 month-old mice.

02 March 2014 3,440 1 View

Problems with Abeta measurements?

Could somebody tell me if the using of the Nanodrop 2000 could be good to measure Abeta concentration after oligomerization in a sample prepared using DMSO and Neurobasal?

09 October 2013 2,507 2 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View