Hi scientists: Does anyone know which chemical can be used instead of PTU (N-Phenylthiourea/phenylthiocardamide) to inhibit melanogenesis in insect hemolymphs .
http://www.sigmaaldrich.com/catalog/product/aldrich/p7629?lang=en®ion=NL
Depends on what is being done-simply diluting hemolymph in a low pH buffer (pH~ 5.0 or anticoagulant buffer) or something like ascorbic acid will inhibit melanization.
Another option is to use Phenylthiourea (PTU, 1 mM) is a good inhibitor of phenoloxidase and is often used in insect plasma to prevent melanization. Example reference is PMCID: PMC3656302
You can also try with the 2-Hydroxy-2,4,6-cycloheptatrien-1-one (Tropolone)
Hi please try the solution by Azambuja et al. 1991 as
:0.01M ethylenediamine tetraacetic
acid, 0.1M glucose, 0.062M NaCl, 0.026M
citric acid, pH = 4.6
good luck,
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