Today we prepare RIPA lysis buffer as following: 

5 ml 1 M tris-cl (PH to 7.4)

30 ml 5 M NaCl 

5 ml 20% Triton-X

5 ml 10% Sodium deoxycholate

0.5 ml 20% SDS

50 ml ddH2O

I wonder what is the wrong that may causes a clear white clumps after adding the Sodium deoxycholate specifically, following the above sequence!

More Ibrahim Elsharkawi's questions See All
Similar questions and discussions