Today we prepare RIPA lysis buffer as following:
5 ml 1 M tris-cl (PH to 7.4)
30 ml 5 M NaCl
5 ml 20% Triton-X
5 ml 10% Sodium deoxycholate
0.5 ml 20% SDS
50 ml ddH2O
I wonder what is the wrong that may causes a clear white clumps after adding the Sodium deoxycholate specifically, following the above sequence!