in Simplified X-ray film method for detection of bacterial volatilization of mercury chloride by Escherichia coli, When i read the abstract, i thought it was about Hg0 detection using x-ray film. But the result says that the foggy area was formed because of Mercury Chloride or HgCl2. I'm confused, since we add HgCl2 into the medium and as far as i know, they were using Mercury Resistant E. coli that will transform Hg2+ into Hg0. And also, in other research they were using this method as a confirmation method of mercury volatilization by the isolate
Article Simplified X-ray film method for detection of bacterial vola...
Article Characterization and potential application in mercury biorem...
I thingk that x-ray film detect Hg0. If no HgCl2 no Hg0 could produced.
Thanks for the answer. I think so too, but when i read the result it says that they were detecting HgCl2 through x-ray film. That's why i'm confused.
? I could not found that kind of description. Maybe you'd confused the expression. Anyway hope good results!
Hg0 reacts with the Ag1+, reducing it to Ag0. It is just a redox reaction, and not specific.
What the author comments in several paragraphs is that:
- some compounds may cause reaction even in absence of Hg2+
"Cysteine, glutathione, mercaptoethanol, and bovine serum albumin formed foggy areas on X-ray film even when Hg2+ was absent."
- Some gases from culturing can have the same effect
"However, foggy formations were observed for all volatilizing
strains after reaction for 24 h at 35°C even when
mercury-free nutrient broth was used as the volatilization
buffer. High reaction temperature and long reaction time
also caused fogging by the non-volatilizing strains. These
results suggest that Ag+ contained in X-ray film can be
reduced by various gases released by bacteria, as in the
former case, or from thioglycolate, as in the latter."
- the vapours produced from volatilising strains can be so abundant that can diffuse to close wells. This may give a false positive in a non-volatilizing or non-HgCl2 wells if they are close to those with a strong activity
"The large amount of mercury vapor produced by resistant
strains sometimes formed faint fog in the areas on the
microplate where sensitive strains had been inoculated. This
fog may have been caused by excess amounts of mercury
vapor which could not be trapped in one spot on the X-ray
film. Hence, the inoculation of bacteria onto the microplate
should be done with plenty of space between each inoculated
spot."
Perfect example of why you always have to run controls
Is there any chemical that we can add to vocalization mixture as positive control?
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