I just finished up a western blot with two specific antibodies (one of which was for tubulin) where, when reading on a 700/800 fluorescent Odyssey scanner, my results look the same as my Coomassie stained gel. I appear to be seeing total protein. There were a few mistakes leading to this, one of which was using secondary antibodies for the wrong wavelengths (488 and 633), but even after stripping the membrane and then blocking again (5% milk in TBST) all the images look the same. They all look like the Coomassie stained gel. I can't come up with a good reason for this; is there something obvious that could be causing this?