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Questions related from Mariam Ahmed Galal
Aslamo Alikom/Greetings everyone, I've been working on MCF7 cancer cell line, and I would like to study their response to cyclophosphamide in vitro. I was wondering what's the best solvent to...
09 November 2023 3,684 1 View
I've been working on MCF7 cell lines for nearly 7 months now. Today while checking one of the flasks, I found its media color dark green and all cells floating dead. I've trypsinized this flask...
24 May 2023 1,336 0 View
Aslamo alikom/Hello I'm planning to do MTT experiment this week but unfortunately, we ran out of phenol-free media (PFM), which is added to MTT reagent for nearly 4 hrs. As it'll take awhile to...
07 May 2023 441 3 View
Aslamo Alikom (Greetings) Everyone, I've MTT results for the cell viability after an exposure to an inhibitor alone and combining it with another drug. Now I wanna see how the other drug affects...
04 March 2023 3,115 4 View
Salam (Greetings), I'm working on Metformin and wanna test its effect on some cell lines when combined with other drugs, which are mainly soluble in DMSO. I've looked up metformin solubility...
10 February 2023 9,988 2 View
I'm assessing the effect of different doses of various drugs on Breast cancer cell lines. All drugs were dissolved in DMSO. Using MTT assay, the impact of some drugs on proliferation was...
01 February 2023 3,003 5 View
Hi (Salam), I'm studying the expression of some proteins in cell lines using LC-MS. In order to do so, I'll go with the "bottom-up" approach. After selecting the suitable signature peptides, I...
30 October 2022 1,369 2 View
