Mariam Ahmed Galal

9 Questions 6 Answers 0 Followers

Questions related from Mariam Ahmed Galal

Can I incubate my membrane with more than 1 antibody simultaneously for WB?

Aslamo alikom/ Greetings everyone, I'm conducting a western blot experiment (8% SDS gel) and I wanna test 2 proteins, one is 95 KDa, and another is 35KDa. The antibodies I've for both are mouse...

26 January 2024 7,615 2 View

Which non-linear Regression option shall I use to calculate IC50 using Graphpad prism?

Hello/Salam Everyone, I'm using GraphPad prism to calculate the IC50 of some drugs in cancer cell line and impact of drug B on the IC50s of these drugs. As per my calculation method, to conduct...

21 January 2024 7,490 0 View

What's the best solvent for Cyclophosphamide Monohydrate in cell culture?

Aslamo Alikom/Greetings everyone, I've been working on MCF7 cancer cell line, and I would like to study their response to cyclophosphamide in vitro. I was wondering what's the best solvent to...

09 November 2023 3,691 1 View

What can possibly cause the media to turn dark greenish?

I've been working on MCF7 cell lines for nearly 7 months now. Today while checking one of the flasks, I found its media color dark green and all cells floating dead. I've trypsinized this flask...

24 May 2023 1,345 0 View

What substitutes phenol-free media in MTT assay?

Aslamo alikom/Hello I'm planning to do MTT experiment this week but unfortunately, we ran out of phenol-free media (PFM), which is added to MTT reagent for nearly 4 hrs. As it'll take awhile to...

07 May 2023 446 3 View

Do I have to normailize my data to calculate IC50 using Graphpad prism?

Aslamo Alikom (Greetings) Everyone, I've MTT results for the cell viability after an exposure to an inhibitor alone and combining it with another drug. Now I wanna see how the other drug affects...

04 March 2023 3,124 4 View

Metformin Best Solvent: Water or DMSO?

Salam (Greetings), I'm working on Metformin and wanna test its effect on some cell lines when combined with other drugs, which are mainly soluble in DMSO. I've looked up metformin solubility...

10 February 2023 9,995 2 View

How to rule out the DMSO effect in drugs?

I'm assessing the effect of different doses of various drugs on Breast cancer cell lines. All drugs were dissolved in DMSO. Using MTT assay, the impact of some drugs on proliferation was...

01 February 2023 3,010 5 View

How to select a suitable isotope to label certain amino acid in a signature peptide for proteomics using LC-MS?

Hi (Salam), I'm studying the expression of some proteins in cell lines using LC-MS. In order to do so, I'll go with the "bottom-up" approach. After selecting the suitable signature peptides, I...

30 October 2022 1,375 2 View