Using a chlorophyll fluorometer (Mini-PAM; Walz; Effeltrich, Germany) I plotted ETR vs PPFD curves. What software / r package/excel sheet can I used to fit this data to a rectangular hyperbola model or other models.
Most literature focuses on fitting gas exchange data, not fluorescence.
Can the same models/excel sheets as created in Lobo et al. 2013 (Fitting net photosynthetic light-response curves with Microsoft Excel - a critical look at the models work?)
Suggestions are welcomed!
Thank you.
You can use GraphPadPrism (PC & Mac), but it is not free (perhaps now there is a free lite version, at least they have a demo; see https://www.graphpad.com/scientific-software/prism/).
Dear Joshua,
If you are fluent in R use (not my case unfortunately):
Technical Report Phytotools: Phytoplankton Production Tools
The Ritchie paper is really woth reading for your very initial question: Article Fitting light saturation curves measured using PAM fluorometry
You will need to have at least some real measurements of dioxygen evolution and / or CO2 incorporation to translate rETR as true production values.
And in my own experience, @Raymond J Ritchie in Researchgate is certainly able to give you precises answers.
Hope this will help,
Christophe
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