I am working with SKOV3 cell line and EDTA is not working on it.
Thanks in advance.
I have recently started working with heterochromatin . I am trying to quantify the H3K9Me levels but i am failing to do so. Sometimes i see faint duplex band . I do TCA Lysis and then follow up...
22 May 2024 2,071 2 View
In the Cartesian Corredinate commonly the direction of the electric field is towards the Y axis and magnetic field is towards the Z axis and the propagation is towards the X axis of EMR. Here, I...
25 September 2023 5,340 2 View
(Metal halide Perovskites)
14 September 2023 9,957 0 View
14 September 2023 7,623 0 View
I have been trying to run a DPV experiment using modified Glassy carbon electrode as the working electrode, Pt as the counter and Ag/AgCl as the reference. Before modifying the electrode, I have...
01 August 2023 8,059 0 View
I have been performed the multinomial logistic regression model in SPSS. The goodness of fit shows that the model fits the data. Based on my literature study, there are several methods can be...
30 July 2023 9,281 5 View
I was discussing with the students in my class on whole circle bearing and quadrantal bearing. They asked me that whether quadrantal bearing and reduced bearing is same or different. I had...
28 June 2023 5,875 4 View
I am trying to induce callus from woody plants grown in an arboretum. We have tried sterilizing explants using bleach but we are unable to get rid of funal contaminations. The explants are woody...
22 April 2023 1,704 2 View
Kindly help.
24 March 2023 7,328 3 View
I am doing edge detection of microcell images directly taken form microscope. One image has 25 microwells containing the cells. I am cropping the 25 automatically and trying to do edge detection....
06 December 2022 3,933 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Is this circle one of the rings?
03 August 2024 5,988 0 View