Can I use FLEXBAR to detect and remove barcodes out of my short reads without demultiplexing ?

Hello, I have demultiplexed my short reads and I have removed the Illumina adapter left in the sequences and now I need to remove the barcodes used by the sequencing centre from my reads. I have...

22 February 2021 9,873 3 View

Does SSMPCA toolbox (scanvp) provide a module to perform the bootstrapping procedure to estimate the reliability of the covariance pattern?

I'm learning to perform the multivariate covariance analysis with SSMPCA toolbox (scanvp/ssmpca, www.feinsteinneuroscience.org). The step-by-step procedure to obtain covariance pattern and...

08 February 2021 2,383 1 View

Has anybody used the Chicken anti-mCherry antibody (ab205402) in immunohistochemistry?

I'm looking for a primary antibody to detect tdTomato in spinal cord slices of mutant mice. I know that the most widely used antibody for this purpose is the mouse anti-RFP from Rockland, but I...

02 February 2021 5,515 3 View

I am culturing murine bone marrow derived mesenchymal stromal cells, what are these cells I am observing?

Dear all, I am isolating mesenchymal stromal cells (MSCs) from the bone marrow of adult C57BL/6 mice. The culture conditions are: -IMDM + L-glut, 20% of 50:50 FBS:HS, 1% P/S -Medium replaced each...

24 January 2021 7,223 3 View

I am inducing ostegenic differentiation in murine bone marrow derived mesenchymal stromal cells, what are these cells I am observing?

Dear all, I am isolating mesenchymal stromal cells (MSCs) from the bone marrow of adult C57BL/6 mice. The culture conditions are: -IMDM + L-glut, 20% of 50:50 FBS:HS, 1% P/S -Medium replaced each...

24 January 2021 3,678 3 View

How can I create a custom corpus for text mining with nltk/spacy?

Hi, I am quite a newbie with python, and I need to run some text mining analysis on 100+ literary texts in German, which I have stored as individual txt files in a folder. They are with the scheme...

20 September 2020 4,762 3 View

Which rapid in-line/on-line method could be used to ensure extraction yield for instant coffe and how to calibrate it on chemometric methods?

There is a need to ensure extraction yield of the soluble coffee process, thus we need a method to analyze carbohydrate degradation products produced after hydrolisys of polysaccharides due to...

11 March 2020 3,308 2 View

How high can be the solar radiation measured outdoor in Australia?

Dear all, I am using a weather station to measure some environmental parameter in the city of Sydney, pedestrian level. In summertime, the global solar irradiation (horizontal) returned by the...

08 March 2020 7,599 6 View

Anyone having problems with low DNA concentrations after RNase A treatment (during DNA extraction)?

I am currently testing the GeneAid DNA extraction kit on abalone (Haliotis iris) attachment muscle tissue samples and it was working pretty fine, until I added the RNase A treatment (as stated in...

26 January 2020 3,019 1 View

HPLC for AAV purification?

Hello, I'm looking into buying a new HPLC machine for purification of AAV preps. Has anyone had a good (or bad) experience with a particular model? Thank you.

22 January 2020 4,024 6 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

12% ledeburitic cold work tool steel, etched for revealing retained austenite, any help for clarifying?

Hello everyone. Given microstructure image belongs to a 12% ledeburitic cold work tool steel (annealed and tempered). The sample was etched with picral (100 mL ethanol and 4 g picric acid). I...

02 March 2021 4,119 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View