What can be the appropriate amount of tween 80 in nanoprecipitation method if polyme is 40 mg, organic phase is 10 ml and aqueous phase is 20 ml?

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Staining of foam cells derived from PBMC differentiated to macrophages. Does anyone have experience with this type of assay?

Hi everyone, I am trying to induce foam cells in macrophage differentiated from PBMC. Which type of staining do you prefer to use? Nile Red or Oil RED O ? What concentration and timing of ox-LDL...

01 July 2024 8,286 0 View

Which negative control do you recommend for In vitro Foam Cell Formation Assay?

Hi everyone I have to perform the Foam Cell Assay with OIL RED O staining. Can you recommend a compound that inhibits LDL oxidation? Also, can anyone recommend oxLDL to use?

14 May 2024 6,404 1 View

Can I transfect differentiated cells?

In particular using a DNA plasmid transient transfection on differentiated skeletal muscle cells?

29 April 2024 9,064 2 View

Research article about minors community?

i need some information to build a thesis about minors community

22 April 2024 1,081 0 View

What do you think I'm doing wrong?

Hi everyone, I differentiate THP-1 in macrophages with PMA 48h + 24h rest. After treatment with LPS I would expect an increase in ROS. But this increase compared to control I cannot get. I have...

25 March 2024 4,797 6 View

Which statistical test should I use?

Hi, I need some information. After collecting data from various experiments, I am proceeding with a statistical analysis. Since I work with frequencies, I was told to use the chi-square test....

20 March 2024 5,496 4 View

When does the Griess colorimetric assay produce yellow instead of pink?

I am using the Nitrate/Nitrite Colorimetric Assay Kit from Cayman. When I used the nitrate+nitrite determination assay with nitrate reductase on the same samples' supernatant, it turned pink. When...

18 February 2024 3,338 0 View

Hello there, any suggestion to calculate equilibrium constants without NMR variable T?

If we have an host-guest complex, in water an equilibrium occurs. So you can have ComplexGuest + H2O ComplexFree + GuestFree. So I can calculate equilibrium constant, but in the most of...

31 January 2024 335 1 View

What medium can I use (instead of RPMI) on differentiated THP1 if my goal is to measure small changes in nitrate levels?

I differentiated THP1 cells into macrophages. Subsequently, macrophages will be treated with substances. Next, the Griess Reagent test will be used to determine nitrate levels. The Griess kit...

24 January 2024 9,551 1 View

Why are my articles linked to another account?

I've registered on ResearchGate today but I can't upload my articles because they are linked to another account with the same name: Giulia Errico, Zurich University of Applied Sciences,...

29 October 2023 1,270 1 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Nano Orange protein quant on the Quantus fluorometer?

Is anyone here using the Thermo Fisher Nano Orange kit for protein quantification, and detecting the fluorescent signal on the Promega Quantus? We are struggling to make sense of the standard...

05 August 2024 8,946 3 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View