Fluro-Jade C, TUNEL, caspase-3, bielschowsky silver stain, gallyas silver stain

thank you monika mam for your answer...can you give the detail procedure of these staining if you have any??

of course, i can write it for you this week, if it is ok :)

Thank you mam..it will be very helpful for my work if you give procedure

once again thanks Monika Závodská

According to the type of neurodegenerative disease.

1-For neuronal degeneration

presynaptic growth-associated protein B-50 (also known as GAP-43, neuromodulin, F1).It's a marker for mossy fiber sprouting associated with neuronal damage as in hippocampal sclerosis.

2-Multiple sclerosis: myelin stain, luxol fast blue.

3-Axonal injury: ß-amyloid precursor protein.

4-Gliosis: as in multi infarct dementia, GFAP

5-Neuropil and axons are well delineated by silver stains.

6-Microglia by CD68

7-Apoptosis by caspase 3.

The pruchures of these products are available online with the detailed techniques for each.