I am going to treat animals with the p38 inhibitor SB203580 and the JNK inhibitor SP600125. I'm looking for a vehicle that can dissolve these drugs without be toxic for the mice. Anyone can help me?
Try dissolving your compound first in a 50:50 DMSO:corn oil mixture. From there, dilute to your desired dosage (while also making sure that the final concentration of DMSO is
Primary solvent for these reagents may be DMSO. Then, you can use PBS as secondary solvent for subsequent injection to mice. You should check to inject no more than 25 ul DMSO per one animal. LD50 for DMSO in mice is 6.2 ml/kg.
Usually i don't trust to use DMSO or EtOH to vehicle something in animals. Try with corn oil, I used that for hydrophobic molecules.
For DMSO, you can inject it to mice, but be sure to be around 1% v/v before to inject it.
Usually DMSO that is dimethyl sulfoxide is a solvent in which most od polar and non-polar compounds get dissolved. To minimise the toxic effect for mice if ant you can try from low conc. of solvent that is 1% and onwards confirming that solute get dissolved. To check that whether DMSO is toxic you can inject first only DMSO in mice in different concentration, and get a max. concentration at which it will not be toxic at all. This group will also act as DMSO control.
DMSO, ethanol, CMC, saline or even water, depending on the solubility of the drug in these solvents. Olive oil, castor oil and palm oil are also used since they show minimal toxicity. However, the choice of vehicle depends on the polarity of drug. And invariably, you still need to keep a vehicle control group in the study.
Dear,
You can try the combination of PEG 4000, N,N-dimethyl acetamide, normal saline.
These kinase inhibitors are recommended to be solubilized in DMSO. As recommended, you could use DMSO for injection but I would dilute the solubilized compound in saline or PBS for injecting it into mice.
If you find LD50 of your inhibitor, then you can choose range of solvents for effective introduction of your drug.
Try dissolving your compound first in a 50:50 DMSO:corn oil mixture. From there, dilute to your desired dosage (while also making sure that the final concentration of DMSO is
Hi tried to dissolve the drug in DMSO and then I diluted it in PBS to have a final DMSO concentraion < 5% but the drug precipitates instantly! What can I do? Did you ever have this problem?
dissolve in 10% DMSO + 20% Cremophor EL +70% normal saline
Check Journal of Medicinal Chemistry, 2010, 53, 7316–7326
DOI: 10.1021/jm100607r
but i found that my drug was inactivated by 20% cremophore solution, i have check both invitro and invivo, why can you plaese give me suggestion? @Mohane Selvaraj Coumar
Dear Ms Marrocco
A more accurate answer to your question would require more information on some aspects such as:
1) What is the chemical nature of the compound you intend to test
2) What is the route of administration of the compound? Intraperitoneal? oral?, etc
3) Among others
I think it is important that you take into consideration the physicochemical parameters that characterize the compound that you intend to test since these will undoubtedly condition its solubility, its stability and therefore its effectiveness.
I am also facing the same problem. My biologists have asked me dissolve the compound in Tween 80 but it is not working. Could you please suggest?
Dear all,
I find this discussion helpful for me.
The drug I want to use for mice (ip daily injection) it is soluble in water. I guess I can not inject the drug in just water.
That's why I would really appreciate if you could suggest me how I could dissovle the drug in. Is this a good option: drug of interest + 50% polyethylene glycol + dH2O?
I hope to hear back from you.
Thank you,
Anna
If it is soluble in water, I would dissolve it in a steril phyosological solution.
Polyethylene glycol is for drugs non soluble in water
sorry. i want to ask. I'm using plant crude extract as treatment but it cant mix well with corn oil only. thus, i added 3% of DMSO. Then, everything going well. after I sacrifice the mice, I found out there was lesions in control group (corn oil mix 3% DMSO). Is it possible outcome?
Dear Ms Marrocco, in such case, have you tried beta-cyclodextrin, it could be a solution
Article Solubilizing Excipients in Oral and Injectable Formulations