Ravi Lokwani

4 Questions 8 Answers 0 Followers

Questions related from Ravi Lokwani

What is the reason of getting a negative median fluorescence intensity for low expression marker in flow cytometry ?

Hi I have a marker with dim cell surface expression but brighter than FMO but what I am seeing is when I am calculating median fluorescence intensity on flowjo it's giving me a negative value but...

03 November 2022 6,045 2 View

When we report MFI in manuscript or any document, so we mention MFI of positive population only or do we mention MFI of entire population?

There are few antibodies like CD54, CD88, for which only a fraction of my neutrophil population is positive and not all. So should I report the MFI of only positive population or MFI of entire...

07 February 2018 8,140 3 View

CCR3 on neutrophil?

Hi I was trying to separate my eosinophil population from neutrophil by using a BD CD193 (CCR3)-BV510. But it showing positive expression for all of my granulocytes. What could be the reason for...

07 July 2017 3,342 4 View

What is the best agent and way to stimulate neutrophils?

I tried 100nM of PMA on 200 thousand neutrophils and incubated 30 minutes (15 minute in incubator and 15 minute after adding PI at room temperature) and all my cells died after that. I am looking...

17 February 2017 8,371 7 View