I want to purchase a pre designed siRNA to silence my gene. So, by searching websites of companies such as Thermofisher, I have found a list of 10 siRNAs for my gene of interest. They differ in the location on the mRNA that they are targeting, given that my target gene consists of only one exon (there are no introns). So, what are the criteria based on which I can choose the best siRNA for my gene.
In addition, what are the best biotechnology companies that provide RNA interference products? I found a lot of papers using products from GenePharma, a company based in China. So, for those who tried RNA interference products from this company, what is the quality and efficiency of silencing of its siRNA products?
Thanks in advance
I know that Sigma-Aldrich and Thermo-Scientific have it. You could try them
We also use siRNA from Thermo. Usually I pick the one that has been used in publications.
The Targefect siRNA kit works very effiiciently for transfecting siRNA even into very difficult to transfect cell types, also works well for co-delivery of siRNA and plasmid DNA and has been used in several publications (selected list of citations and product link is given below
https://targetingsystems.net/pdf/siRNA.pdf
https://targetingsystems.net/citations.php#
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