What are the standard methods used for the estimation of secondary metabolites from medicinal plants?

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Olukayode Solomon Ajayi

I suggest you first carry out qualitative phytochemical test on the extract of the medicinal plants to give you first-hand information of the various secondary metabolites in them and then we can decide which to be used for a particular phytochemical estimation.

Pierre André De Souza

HPLC-MS-MS (if you already known what secondary metabolites you want) and CG-MS (the most comun phytochemical estimation)

Dr. Tresina Soris P

Respected Sir,

As Olukayode Solomon Ajayi Sir said, you have to perform qualitative preliminary phytochemical screening followed by HPTLC, GC-MS/LC-MS analysis and finally extraction, isolation, purification, identification and structural elucidation of the phytochemical constituents (compound isolation).