My Rossette pET E. Coli suddenly turn pink, particularly with one 100 and one 1000 Neat Dilution ?

Dear Colleagues I am working on genes for more salt tolerance, however, suddenly I observed that my colonies in plate turn pink, particularly, with low population of E. coli Rossette pEt... I...

07 August 2019 7,483 2 View

How monocotyledonus plants experience secondary growth.However, their fascular bundles devoid of cambium?

Stem thickening is achieved with out vascular cambium

09 October 2017 2,565 2 View

How to determine Nitrate in plant tissue by cardy chlorometer

04 May 2017 440 3 View

What are the risks of consuming gene modified crops?

07 August 2016 1,237 8 View

Why our wheat flour for bread required amendment with pulse flour and others?

07 August 2016 8,909 2 View

Why Alkaloids are formed in plant cells?

06 July 2016 6,431 6 View

Why crystals are formed in plant cells, particularly in parenchymatus?

06 July 2016 3,000 3 View

Why shade plants manifested longer internodes and thinner leaves?

When plants of the same cultivar cultivated in high populations or grown in shade their internodes being longer and their leaves thinner and of higher area than those cultivated in natural sun light.

05 June 2016 5,507 1 View

What is the mechanism by which pH facilitate metabolic reactions?

In most chemical analysis for determination of a given compounds, protocol is usually, recommended a given pH if we do not confined to this pH we will not accomplished the desired...

05 June 2016 676 7 View

Are there any differences in the biomass productivities betwwen plants of the same cultivars gron in tree shades and building shades?

05 June 2016 6,553 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

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02 March 2021 3,060 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View