Fluid mechanics in a solid channel?

I have a fluid mechanics questions. Suppose that we a submillimeter needle that is already inserted in the soft tissue. water can run through this needle if the pump is on. suppose we turn the...

03 April 2019 7,287 3 View

What does negative shear modulus mean in Ogden fit to data?

I am fitting Ogden model to my stress-stretch ratio data (obtained from compression test). The fit looks good but the predicted shear modulus from ogden fit is negative. Does it have any meaning?...

02 March 2019 9,349 7 View

What are the properties of soft tissue at failure?

In the attached paper and all other paper in needle insertion, the people modeled the tissue using an Ogden model. While Ogden model is an "elasticity" model, and the tissue goes through failure...

02 March 2019 9,943 5 View

Finite element analysis of depth of cut of water-jet as a function of water velocity?

I want to predict the depth of cut that a submillimeter water-jet cuts in tissue as a function of water velocity with FE modeling. Can anyone help me with correct resources to do it? I am not an...

08 September 2018 8,662 1 View

Does the pumps show pressure drop in system?

Considering that pumps create flow and not pressure, if a pump runs water through a pipe into the air (high velocity water), is it true to say that: the pressure that pump shows - atm pressure =...

04 May 2018 4,605 5 View

What is the stagnation pressure of a jet of water impinging on a solid in case of pressure loss in pipe?

suppose that we have two pipes with different diameters one after the other. the jet of water coming from the last pipe is impinging on a solid, if we have pressure drop in pipes (I have...

03 April 2018 7,611 11 View

Do i need a bi-directional load cell?

I am using a load cell at the base of the needle to measure the forces on the needle applied from the tissue that the needle is inserted into. However I also want to record data while retracting...

03 April 2018 6,183 2 View

Call for collaboration on a project

I have recently developed a closed form mathematical model for depth of cut of water-jet in soft tissue as a function of tissue parameters (toughness, and constitutive response) as well as...

01 January 1970 2,562 4 View

Publishing with intechopen

01 January 1970 3,216 11 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View