I want to clone an E.coli gene in pMALp2X vector together with trp promoter and rrnB-T2_terminator. Will this construct be enough to express the gene of my interest when inserted in any non essential region of pMALp2X vector.
Your resulting construct will express your protein but it is not a ideal construct. The problem is that you will introduce the repeat sequence in pMALp2X vector by doing that way and any repeat sequence is keen to be deleted (recombination) during the vector replication.
If you want to do co expression, there are some dual expression vectors available such as pRSFDua that athat are much more efficient than the self constructed ones, or if you definitely need to do so you can clone your pMAL expression cassette into vectors that do not use the identical trp promoter and rrnB_T2_Terminator.. Vector modifying is time-consuming and usually less efficient than the commercial ones.
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