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Questions related from Arunangshu Das
I have been setting up overlap extension PCR to join four fragments of size 500, 2140, 1300 and 500 base pairs. I am adding equal amount of DNA in molar ratios and using Taq Polymerase with traces...
13 July 2022 4,192 0 View
I want to transfer a whole mutant GFP library under arbinose inducible promoter on a pBAD backbone to pMAL p2x backbone under tac promoter? What is the best method for this? What is typical % of...
05 March 2019 4,507 2 View
Please if any body have this book provide me. I need it for further planning of my research.
07 August 2018 8,674 2 View
I want to clone an E.coli gene in pMALp2X vector together with trp promoter and rrnB-T2_terminator. Will this construct be enough to express the gene of my interest when inserted in any non...
09 September 2016 7,546 3 View
Need this ionic liquid at New Delhi. Any national or international vendor who can supply this.
17 May 2016 393 14 View
I have a DNA sequence for an enzyme available in Human. I took the sequence and generated a set of codon optimized sequences using random codon optimization for expression in E.coli. Now what are...
20 November 2015 6,890 3 View