Arunangshu Das

9 Questions 11 Answers 0 Followers

Questions related from Arunangshu Das

If the effective buffering range of Tris is between 7 to 9 why do we use Tris pH 6.8 in stacking for SDS page gels?

Is is possible to shift to better buffers like MES for this purpose? Will these improve our results?

18 August 2023 9,029 0 View

Why it is problematic to obtain PCR amplification using the product of overlap extension PCR as template and the same terminal primers?

I have been setting up overlap extension PCR to join four fragments of size 500, 2140, 1300 and 500 base pairs. I am adding equal amount of DNA in molar ratios and using Taq Polymerase with traces...

13 July 2022 4,202 0 View

Can any one provide us genomic libraries constructed from bacterial consortia belonging to different ecological niche such as hot spring, salt marsh?

We need genomic libraries made on vectors compatible for proagation and expression in E.coli. Any researcher who have such libraries can help us with it. We will be happy to bear shipping charges...

08 March 2022 2,882 0 View

How to transfer a mutant library harbored on a plasmid under arabinose inducible promoter to different plasmid having tac promoter?

I want to transfer a whole mutant GFP library under arbinose inducible promoter on a pBAD backbone to pMAL p2x backbone under tac promoter? What is the best method for this? What is typical % of...

05 March 2019 4,510 2 View

Does any one have books 'Energetics and Kinetics in Biotechnology' by J.A.Roels?

Please if any body have this book provide me. I need it for further planning of my research.

07 August 2018 8,683 2 View

What are the requirements for trp promoter to work in E.coli. If trp is the promoter can we use rrnB_T2_Terminator as a terminator with trp promoter?

I want to clone an E.coli gene in pMALp2X vector together with trp promoter and rrnB-T2_terminator. Will this construct be enough to express the gene of my interest when inserted in any non...

09 September 2016 7,551 3 View

Any have any idea which vendor can provide choline dihydrogen phosphate cas no. 83846_92_8 in India?

Need this ionic liquid at New Delhi. Any national or international vendor who can supply this.

17 May 2016 397 14 View

I am a chemistry graduate. But I need a protocol which I can simply follow to knock in genes in E.coli?

Being a chemistry student I do not know much molecular biology. However I need to knockin some genes in E.coli. Please provide me a simple effective protocol and some papers through which I can do...

04 May 2016 2,987 1 View

What are the possibilities that enzymes coded by codon optimization different sequence for the same gene differs from one another and at what level?

I have a DNA sequence for an enzyme available in Human. I took the sequence and generated a set of codon optimized sequences using random codon optimization for expression in E.coli. Now what are...

20 November 2015 6,895 3 View