What are the recent detailed assay protocols for antioxidants in plants?

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Johansen test question on the number of cointegration relationships?

Hello everyone

01 August 2024 6,431 4 View

Granger causality construction for VECM model?

Dear people,

24 July 2024 1,252 2 View

VECM Model: Positive/insignificant CointEq1 ?

Hello everyone, I am conducting

22 July 2024 8,085 11 View

Optimal lag length: what is the maximum lag length I should put?

Dear Pe

20 July 2024 9,703 6 View

What are the links between diets and chronic metabolic diseases?

is there any link(s) between insulin resistance and ghrelin resistance?

02 June 2024 9,844 2 View

How do I investigate hot spot temperature prediction of transformers?

I want understand how to go about this in my project

28 February 2024 7,802 3 View

What sorting techniques should be used to separate T24 bladder cancer cells and myeloid cells ?

I am currently working on the T24 bladder cancer line and I am co-culturing for 72h with immunosuppressive myeloid cells generated in vitro. After 72h of co-culture, I want to isolate T24 cells by...

15 December 2023 2,893 0 View

Which LLM best supports Transparency and Reliability for AI-enabled Educational Technology?

Many LLMS provide the algorithmic rules for generative AI. However, there is still a problem with the transparency and reliability of these LLMs. Higher education has witnessed a decreased...

13 November 2023 9,133 4 View

How do i publish an article?

I would like to publish an article

27 July 2023 7,780 2 View

Boarding school meals?

I need a project work on nutrient composition of boarding school meals

13 June 2023 7,470 5 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

P. Pardha-Saradhi

You may follow the protocols used by us earlier. They are reliable. I am attaching two of our publications for details.

Clement Ogunkunle

@Pardha-Saradhi. Thanks so much, I will go through the two paper ASAP

Ajay Sharma

this paper may help you..

@Sharma, thanks so much.

Alok Nahata

The attached links would be useful for you.

http://www.ncbi.nlm.nih.gov/pubmed/20870416

http://www.ncbi.nlm.nih.gov/pubmed/19121097

Attached paper is based on CAT determination. Hope it helps.

Sujayraj R.S

Kindly find the attachments..Hopefully you might find it useful

Moses Olayemi Akiibinu

P. Pardha-Saradhi gave a useful and considerable advice.

Mital Kaneria

Attached paper is based on in vitro assays...