Ajay Sharma

10 Questions 33 Answers 0 Followers

Questions related from Ajay Sharma

How to characterize the structure of single spot unknown compound isolated from bio-active fraction of plant extract (detailed procedure)?

I am working on Himalayan aromatic plant. i isolated a single spot compound from bio-active fraction of plant extract. Now i have IR, UV-Visible, HRMS, 1H NMR,13C NMR, DEPT - 90 & 135, COSY,...

30 May 2017 4,127 6 View

How to preserve lutein standard for further studies?

I am working with carotenoids mainly Lutein obtained from peels of various fruits. For this i have purchased Lutein standard, which was very costly (1mg- Rs 38.000/- Indian currency). So i want to...

26 December 2016 4,205 4 View

How much of extract and resin (Diaion and Sephadex LH-20) should be taken for better separation?

i want to separate polar fraction of plant extract. For this i want to know how much amount of plant extract should be loaded in the column. Further, for batter separation what should be the...

21 December 2015 1,997 3 View

How to calculate the mg Fe (II) equivalent/g of dry weight in case of FRAP assay?

Standard equation of FeSO4 is y=4.5682x-0.0347. Different concentration used for the preparation of std curve are 60, 120, 180, 240, 300 mg/L. I took 100ul of sample having concentration 1mg/ml...

19 November 2014 2,610 5 View

How does one calculate the results of nitric oxide scavenging activity?

I have calculated nitric oxide scavenging activity with the help of griess reagent as follow:- reagents Phosphate Buffer Saline (PBS) pH 7.4 10 mM sodium nitropruside prepare in PBS (298mg in...

01 October 2014 1,987 9 View

What is the difference between iridoid and iridodial compounds and what are the different techniques used for their separation from plant extracts?

Which is the best method for their identification.

17 August 2014 6,774 3 View

How long should Soxhlet extraction be run in order to have complete extraction of secondary metabolites from plant material?

I want to use Soxhlet method for the extraction of different extracts from plant material. but I don't know how long the Soxhlet extraction Should be run for complete extraction of secondary...

24 June 2014 5,281 15 View

Is there any in vitro method for the evaluation of anti-inflammatory, anti-diabetic, anti-cancer, anti-nociceptive and cytotoxic activity?

I have different extracts of a medicinal plant. This plant have been known for its medicinal potential in traditional medicinal systems. So, I want to evaluate the biological potential of this...

23 June 2014 7,475 13 View

Can we used catachin hydrate as a standard for the estimation of total flavonoid in plant extracts?

I want to evaluate total flavonoid content in plant extracts and I have catachin hydrate but not catachin, rutin and quercetin. So can I use the same as standard in the assay?

19 June 2014 6,000 10 View

Can we dissolve different extracts in different solvents for sample preparation for total phenolics and antioxidant essay?

We are evaluating the antioxidant properties with DPPH method and total phenolics. for this three extracts of dry plant material were prepared viz hexane, chloroform and methanol. For preparing...

17 April 2014 266 2 View