In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Why marketers need a forensic understanding of consumers and a creative, agile mindset?

02 February 2021 6,579 10 View

Can anyone help me to solve this problem?

when i did time history anlysis by using sap 2000 , the program showed the first two step only at .02 and .04 . can anyone help me to solve this problem?

27 November 2020 3,810 2 View

Biotech Startup Research?

26 October 2020 9,698 5 View

While assigning hinges to column and beam in sap2000V14. i get this error in assigning hing .what is the solution now?

when i assign hinges for beam and column before pushover analysis in SAP 2000,. a error is shown related to assigning hinges . How can i overcome from this issue? kindly help me if someone find...

01 September 2020 3,072 3 View

Why CSI Bridge/SAP2000 showing Plastic Hinge in Bridge Pier even if Pier Moment Value has not reached to it's Mp Value?

Hello every one, I am doing analysis of Bridge in which elastomeric bearing is modeled as link element between Deck and pier bent. Pier bent portal consist 2 pier and one cap beam on which...

12 August 2020 8,625 2 View

Which is your preference when analyzing 3D frames; STAAD, ETABS, LUSAS, RISA 3D SAP200 or ROBOT?

In a followup question to what was previously asked, which of the following would you recommend for analysis and performing design checks on 3D frames: (1) STAAD (2) ETABS (3) SAP2000 (4)...

02 August 2020 3,717 11 View

Can anyone suggest a research topic in the project management field?

I am thinking about the hybrid Agile waterfall management approach in construction projects, but i couldn't advance more in my research. I have a background in International Business and...

13 July 2020 2,957 8 View

Can you please suggest literature that talk about how to be adaptable and agile in your qualitative research design?

I would like to read on how people have been adaptable and agile when conducting qualitative research in uncertain environments. Usually typing "agile methodologies" often lead me to work in...

04 June 2020 6,647 1 View

How we could modeling orthotropic membrane elemnt in SAP2000?

I am trying to model steel plate shear walls in SAP 2000. First I should define an orthotropic steel material and define nonlinear parameter of it ( Stress-Strain curvature) in somehow to neglect...

22 May 2020 4,250 0 View

Where to find case studies for teaching and assessing studnets' understanding of agile project mgmt?

I am wondering if there are any case studies used for delivering and assessing students understanding of agile project management Scrum framework. Please feel free to share some of your agile PM...

05 February 2020 3,229 6 View