Hey guys, I did a restriction digest and ran my sample on an agarose gel. Under the UV light, all I can see are the bands of the ladder. A loading buffer was used.
Is this genomic dna or a pcr product being cut?
Is there a bright fluorescence caught in the wells where the samples were loaded?
Do you have a picture we can see?
How did you measure the amount of dna before cutting?
This will happen if you use more than 2ul of loading dye in your DNA sample or if the loading dye isn't vortexed so the glycerol in it is all at the bottom of the tube. Your DNA probably did not stay in the wells and diffused into the TAE buffer.
it happened to me in the beginning. also use at least 100ng dna per sample, 150 max. I also get far better results with inexpensive etbr than any of the "safe" subsitutes. Also always check your DNA concentration with a flourometer or a spectrophotometer.
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