biofilm and quorum sensing genes of E.coli, not used drug but chemical material (Thiophene)
It is not obvious to me why you say there is contamination? Maybe there was no inhibition and the cells grew everywhere?
Do you have negative control lanes (no cells)?
Do you know your medium was not contaminated or maybe your multichannel pipet and/or tips?
Hello,
if even your negative control is contaminated, there could be several reasons for this general contamination, potentially this happened during the incubation, so I suspect the incubator is contaminated. Try disinfecting the incubator or incubating elsewhere.
Thank you very much to all, yes the main cause is the Incubator or Hood, we will change them, although we disinfected everything in alcohol, and everything is sterilized by Autoclave like tips, etc…of course we did negative control of wells and all the procedure precisely but even control is contaminated, the ability of Thiophene to inhibition need to be checked before using in MIC experiment
Aseptic methods to be followed in all microbiological processes ... fumigation may not properly carried out in working area
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