What are the necessary primer for sequencing of Azotobacter spp.?

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Sosanka PROTIM Sandilya

Dear Amin

Please go through this link (http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300003).

Regards,

Sosanka

Sai Shiva Krishna Prasad Vurukonda

If you want to Identify the Azotobacter sp. strains, you can use 16 rRNA gene universal primers 27F and 1492R.