I am going to sequence some isolates that have some physiological and biochemical properties as Azotobacter spp. What primer or primers should I use to sequence those isolates ? Any suggestions are welcome.
Dear Amin
Please go through this link (http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300003).
Regards,
Sosanka
Thank you Sosanka for your link.
If you want to Identify the Azotobacter sp. strains, you can use 16 rRNA gene universal primers 27F and 1492R.
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