Nitrogen fixation, Phosphate solubilization, Root colonization, Hormon production such as IAA, Biofilm and Sidrophore production, Enzyme activity like protease, lipase, cellulase, kitinase
You should notify that all PGPR do not have all of these features, but they have at least one or two
There is lots of papers about PGPR, you can check the review papers of Prof. Bashan , Prof. Rainers etc.
Apart from those traits suggested in previous comments, you may also test ACC deaminase activity, biocontrol properties (direct inhibition of pathogens or priming effect), potassium solubilization, protection against abiotic stresses (drought, salinity, heat, high metals concentration)
See: Menéndez and García-Fraile, 2017. AIMS microbiology, 3(3), 502 and references herein. Article Plant probiotic bacteria: solutions to feed the world
In addition to nutrient solubilization and hormonal/enzymatic growth activity, the adaptability and survivability of microorganisms in a real environment is one of the most important features.
Of course dear Alae Elabed, because the role of beneficial microorganisms is also gaining importance in stress management (e.g., drought, heat, and salinity) and the development of climate change resilient agriculture.
we should check the plant growth-promoting traits of PGPR like phytohormone production, nutrient solubilization like P, N, K, Fe., production of ACC deaminase both under greenhouse and field conditions. The viable count of microbes should also be checked when applied in field conditions at regular time intervals.
That's a very relevant question. For me, the development of a biofertilizer should first of all take into consideration the capacity of the strain(s) to provide nutrients to the plant (nitrogen and phosphorus in particular); also the best strain for a biofertilizer should inhibit plant pathogens and synthetize phytohormones.