What are the most effective cryopreservation protocols for agarolytic bacteria in terms of maximizing cell viability and recovery rates post thaw?

08 January 2025 1 899 Report

I have a consortium of agar-degrading microorganisms on the f/2 agar plate, and they have somewhat liquidated the agar. Now I want to know how to collect the bacterial culture from the plate and cryopreserve it so that there is no agar debris in it that could hinder the cryopreservation process. Are there specific cryoprotectants or freezing methods that are recommended for these types of microorganisms?

Michael J. Benedik

If you want to preserve the consortium (instead of just specific colonies) then I would add some buffer or media to the surface (a few ml) and scrape all the cells off. Mix well and use some aliquot to freeze away. I doubt that a small amount of agar will be inhibitory in any significant way.

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