Unfortunately you will struggle to extract microbial DNA directly from dairy products. The quantity of protein will the main issue but your DNA will contain contaminates which will hinder downstream enzyme based experiments such as PCR, sequencing or endonuclease digestion. I know paraTB takes a long time to grow but isolation onto agar would be the best starting point. 

If you just want to confimr presence you could either try DNA finger printing

http://www.ncbi.nlm.nih.gov/pubmed/22807025

or a RTPCR based method

http://www.ncbi.nlm.nih.gov/pubmed/23976784

Thanks for your replay

although culture is the golden standard, i need faster methods beside it 

 Hi

Please look in attach file 

Thanks Dr. Alaa 

I tried many kits and different ways for extracting total DNA from milk and the only one that worked great with me and got very good quality DNA for downstream microbiome analysis was The DNeasy PowerFood Microbial Kit from Qiagen

I recommend it for anyone working with dairy products specially if you need a high quality low PCR inhibitors DNA