Any idea about break dormancy in a wild-type seed?

15 December 2016 6,550 10 View

Anyone know a free software to do the consensus sequence?

27 October 2016 6,435 4 View

Why some protocol for DNA extraction use Phenol?

04 April 2016 1,891 4 View

How to gate mouse MDSC cells through flow cytometry?

On inducing the mouse bone marrow cells with X compound, there is a change in cells morphology and characteristics. In that case how to gate these cells, as these cells are showing suppressive...

27 January 2021 608 2 View

Can anyone identify this fungus/mushroom from China?

The pictures were taken in Guishan Mts in Yunnan. The fungus is ca. 10-12 cm high.

07 January 2021 6,639 10 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View

Are these IPScs Differentiated ?

Hi everyone, So these are images of IPSCs 2 days post passaging/splitting. I noticed that the overall morphology of colonies are not compact or round and without distinct border. However, at...

28 December 2020 3,503 3 View

Can forward and reverse sequences of PCR products be entirely different from each other?

Dear All, The forward and reverse sequences obtained from the PCR products are not identical at all. Can I still make the contigs for doing BLASTn? Individually both sequences are showing the...

22 December 2020 5,280 5 View

How to perform the SEM, TEM characterizations to study the surface morphology of a Gold-coated PCF sensor?

I have designed an SPR based PCF sensor, which has a TiO2 and Gold coating. A reviewer has asked for the characterizations (e.g. SEM, TEM) to study the surface morphology. The reviewer has also...

06 December 2020 7,210 3 View

How we can isolate" distinguish" between Tyrophagus putrescentiae and T. entomophus ?

If I want to isolate Tyrophagus entomophagus by selective media, as I know they can grow on molds of stored grains,fruits, cheese. Or even in beehives debris. But how can I got only one species...

30 November 2020 9,087 3 View

In this sterile needle biopsy wet-mount, does the motility pattern fit with Oomycete zoospores?

We are examining tissue from a cluster of human and canine patients with a similar pattern of systemic illness of unknown cause. All members of the cluster have evidence of motile zoospore-like...

23 October 2020 4,480 3 View

Can you help me explain these morphological changes in Human fibroblasts?

Hello I have these human fibroblast cells in culture for a while and they look fine with a typical fibroblasts morphology, At passage 8 the culture looks like a heterogenous culture with...

22 September 2020 7,856 3 View

Complete protocol for Fungal Genomic DNA isolation?

Dear all, I am looking for recommendations for an efficient protocol for the extraction of Genomic DNA from several fungal cultures. I have most of the chemicals and equipment used in the process,...

20 September 2020 7,906 7 View