Genetic methods to screen for mutations in plant genes, enable genes and function to be identified. High-throughput screens are needed to be able to identify low frequency mutations. Since photosynthesis has many genes and pathways, what are the components of photosynthesis that are most amendable to identify mutants using different screens?
I think chlorophyll fluorescence can capture the emergent properties of the photosynthesis system and is very fast, so it should be a good tool for screening photosynthesis mutants. Also, a thermal camera can give information on relative leaf cooling, which rapidly captures the emergent properties of stomatal openness and transpiration, and they give information about the stomatal limitations to photosynthesis, which should be coordinated with carboxylation capacity. Traditional gas exchange approaches (a-ci curves, light response curves), give more detailed information, but are time-consuming and not practical for screening large numbers of mutants.
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