Dear Satish,
METHODS TO TEST PERIODONTAL LIGAMENT CELL VIABILITY
Available tests
The Cellomics Cell Viability HCS Reagent Kit(Fischer Scientific, Pittsburg PA,USA) provides high-content screening (HCS)-qualified fluorescence reagents, which help in screening cell viability.
The MTT assay, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide], has been used to test cytotoxicity of reagents and cell viability.
The HCS reagent kits may be more sensitive than traditional assays, such as MTT assays, which rely on late-stage cytotoxic endpoints and can detect neurotoxic effects at lower concentrations than these biochemical assays.
Fluorescein diacetate staining of viable cells
Cell viability can be assessed directly through the presence of cytoplasmic esterases that cleave moieties from a lipid-soluble nonfluorescent probe to yield a fluorescent product. The product is charged and thus is retained within the cell if membrane function is intact.
Strategies to preserve the periodontal ligament cell viability
Avulsion of permanent teeth is the most serious of all dental injuries and one of the few real emergency situations in dentistry. The prognosis depends on the measures taken at the place of accident or the time immediately after the avulsion because the preservation of the cell viability would be significantly higher.
For more on this topic, please see the review article contained in the following link:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3198546/
Hoping this will be helpful,
Rafik
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