I want to perform an experiment with plants i.e. micro algae, hydrophytes and some other terrestrial plants in order to understand the iron uptake mechanisms in the plants and their effect on the plant physiology.
Ok thanks, but i want to know about that its not answer of question and its not much related to that why you making this chuckling you want to show off that you are geneous so...thing like that or .
I simply ask for methods or simple procedure so that we can understand the molecular mechanism of the iron in the plant cell.
Is this any good to you? A comparative study of iron uptake mechanisms in marine microalgae: iron binding at the cell surface is a critical step.
http://www.plantphysiol.org/cgi/pmidlookup?view=long&pmid=23033141
You might find some useful information from the review paper by Dr. Moshe Shener and Prof Yona Chen;-Increasing Iron Availability to Crops: Fertilizers, Organo‐Fertilizers, and Biological Approaches-Soil Science and Plant Nutrition (. 01/2006; 51(1):1 - 17. DOI:10.1111/j.1747-0765.2005.tb00001.x
@ Thanks xiaolan but i have no acess of this paper so please ,if you have this paper please share with me.
In terrestrial plants this has been studied a lot in the 1980s and 90s. The basic message ist that higher plants usually use either a reductive pathway or a phytosiderophore. I suggest that you look up the older papers, e.g.
Marschner, H., and V. Römheld. "Strategies of plants for acquisition of iron." Plant and soil 165.2 (1994): 261-274.
Marschner, H., V. Römheld, and M. Kissel. "Different strategies in higher plants in mobilization and uptake of iron." Journal of plant nutrition 9.3-7 (1986): 695-713.
and the papers that cite them. I think you have a good chance to find them as pdfs via google scholar.
In unicellular aquatic bacteria or phytoplankton, several cyanobacteria can produce a siderophore, while for eukariotic cells it seems that they can take up iron bound to inorganic or organic ligands either via a ligand-exchange reaction to some specific transporter ligand at the cell surface, or rely on reducing the iron in the complex at the cell surface and then taking up the reduced Fe(II). I have studied these processes in a model (Völker C, Wolf-Gladrow DA (1999) Physical limits on iron uptake mediated by siderophores or surface reductases. Mar Chem 65: 227–244). But probably you are more interested in the practical and measuring aspects.
For that the first paper that S. Velivelli cited (and the references in it) is an excellent starting point for your literature seach. A few more important papers are
Hudson RJM, Morel FMM (1990) Iron transport in marine phytoplankton:
kinetics of cellular and medium coordination reactions. Limnol Oceanogr
35: 1002–1020
Maldonado MT, Allen AE, Chong JS, Lin K, Leus D, Karpenko N, Harris
SL (2006) Copper-dependent iron transport in coastal and oceanic diatoms.
Limnol Oceanogr 51: 1729–1743
Butler A (1998) Acquisition and utilization of transition metal ions by
marine organisms. Science 281: 207–210
Butler A (2005) Marine siderophores and microbial iron mobilization. Biometals
18: 369–374
Shaked Y, Lis H (2012) Disassembling iron availability to phytoplankton.
Front Microbiol 3: 123
I hope this helps; but it probably also shows that you should not expect one simple answer yet. The whole issue of iron uptake is still very much active research. And it is not easy to study in the lab, because of contamination issues.
You can ask Moshe about Fe nutrition and get his paper. He did many works on iron nutrition.
His e-mail is [email protected].
it is thought that the rate determining step for Fe acquisition in terrestrial Strategy I plants (dicots and non-graminaceous monocots) is the reduction of Fe at the root surface. Fe must be reduced before root uptake. For Strategy I plants, this reduction step is considered to be done by a root plasma-membrane-bound Fe reductase. Measurements of the rate of this reduction step can be undertaken quite easily on whole root system, or even excised roots by using a Fe source and a Fe2+ complexing agent.
Take a Captain Cook (i.e. look) at;
Grusak MA. 1995. Whole -root iron(III)-reductase activity throughout the life cycle of iron-grown Pisum sativum L. (Fabaceae): relevance to the iron nutrition of developing seeds. Planta 197: 111-117.
Uptake wise is a different story, ion channels are used are regulation of Fe uptake via a channel is not generally believed to be the controlling factor in Fe uptake, but this level of regulation should not be discounted.
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