How to store gel for MS analysis?

Please suggest, how to store a SDS-PAGE gel, after colloidal coomassie staining? I have to do LC/MS analysis, but instrument is not available for few days. can i store the gel? if yes how long? in...

05 June 2017 1,414 5 View

How we can analyze electron density map of cryo EM structure?

11 December 2016 1,965 4 View

Why the protein is not denaturing in SDS PAGE?

I am working with a protein of approx 35kDa, in SDS PAGE the band is coming at approx 72 kDA. If it is in Dimeric form then why it is not coming as monomeric form. Laoding buffer contains 5% BME,...

09 October 2016 8,592 8 View

Had any body done Circular Dichroism studies of ATP bound proteins?

I want to study conformational / structural change in the protein after ATP binding ( I am working with a yeast protein that shows ATPase activity) so I want to see and compare the binding effect...

08 September 2016 4,347 4 View

Does the ratio between CD spectra at 208 and 222 nM Matters?

For Circular dichroism studies, we know that if the negative elipticity at 208 and 222 are more then it would be alpha helical protein. but if it is more at 208nm or more at 222 nm, what does it...

06 July 2016 2,196 4 View

What is the minimum concentration of protein required to get Circular dichroism data ?

06 July 2016 8,317 5 View

Can any one tell me why my RT (reverse transcription) is not working?

I have RNA, isolated from Yeast cells (By TRI (SIGMA) reagent), quality is good after Dnase-1 treatment (on Gel), but cDNA is not formed. MMLV RT is working fine for other strains (RNA samples)....

03 April 2016 9,529 9 View

Does the anti-TAP antibody detect CBP tagged protein (Calmodulin binding peptide)in Western Blotting?

I am using a fission yeast strain which has a Calmodulin binding peptide(CBP) tagged protein.I want to check whether this CBP tagged protein interacts with my protein of interest by CoIP. I plan...

09 October 2015 8,685 2 View

Why does the protein not bind to PVDF/ MCE membranes? Did anybody face this problem in filter binding?

I am doing RNA-Protein interaction exp. I want to see binding by filter binding assay, I used Millipore PVDF 96-well filter plates, with vacuum manifold, but could not see binding, I also tried...

07 August 2015 2,391 4 View

What could be the reason for protein degradation during sample preparation?

During sample preparation for SDS-PAGE, some of my samples are precipitating metimes. They give smear like band on gel, what could be the reason? Heating at 95 for 5min and then cooling on ice for...

01 February 2015 6,885 7 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View