What are the possible applications of these compounds..1-Indanol , Methyl-4 pyridine methanol , 1-Naphthyl)ethanol?
Please suggest, how to store a SDS-PAGE gel, after colloidal coomassie staining? I have to do LC/MS analysis, but instrument is not available for few days. can i store the gel? if yes how long? in...
05 June 2017 1,588 5 View
how to obtain electron density map of a particular cryo EM structure? And how to analyze it? Is it posiible to see a particular amino acid side chain interaction using cryo EM data? If yes, How?...
11 December 2016 2,121 4 View
I am working with a protein of approx 35kDa, in SDS PAGE the band is coming at approx 72 kDA. If it is in Dimeric form then why it is not coming as monomeric form. Laoding buffer contains 5% BME,...
09 October 2016 8,768 8 View
I want to study conformational / structural change in the protein after ATP binding ( I am working with a yeast protein that shows ATPase activity) so I want to see and compare the binding effect...
08 September 2016 4,520 4 View
For Circular dichroism studies, we know that if the negative elipticity at 208 and 222 are more then it would be alpha helical protein. but if it is more at 208nm or more at 222 nm, what does it...
06 July 2016 2,554 4 View
I am working with a protein of approx. 45kDa, I want to take spectra in far UV region, protein is highly prone to aggregation so I am getting very low conc. Please suggest what is minimum conc....
06 July 2016 8,443 5 View
I have RNA, isolated from Yeast cells (By TRI (SIGMA) reagent), quality is good after Dnase-1 treatment (on Gel), but cDNA is not formed. MMLV RT is working fine for other strains (RNA samples)....
03 April 2016 9,707 9 View
I am using a fission yeast strain which has a Calmodulin binding peptide(CBP) tagged protein.I want to check whether this CBP tagged protein interacts with my protein of interest by CoIP. I plan...
09 October 2015 8,816 2 View
I am doing RNA-Protein interaction exp. I want to see binding by filter binding assay, I used Millipore PVDF 96-well filter plates, with vacuum manifold, but could not see binding, I also tried...
07 August 2015 2,542 4 View
During sample preparation for SDS-PAGE, some of my samples are precipitating metimes. They give smear like band on gel, what could be the reason? Heating at 95 for 5min and then cooling on ice for...
01 February 2015 7,061 7 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified
31 July 2024 5,113 4 View
After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...
29 July 2024 9,753 3 View
I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...
24 July 2024 8,696 0 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
Thanks
22 July 2024 1,143 3 View
Suggest the best and shortest route for the chemical synthesis and and green synthesis of magnetite(Fe3O4) The chemical synthesis should contain the least dangerous chemicals along with full...
21 July 2024 2,476 2 View
Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...
19 July 2024 2,155 0 View