RCP : Representative Concentration Pathways
I am extracting RNA from human adipose tissues using the TRIzol method, following the attached protocol for tissue extraction. The problem is that the 260/280 ratio of my samples is low in most...
10 June 2024 4,614 3 View
please tell us my research gate id.
03 June 2024 3,623 0 View
substrate temperature for sputtering deposition method.
12 March 2024 6,970 3 View
I am growing my HepG2 cells in low-glucose DMEM (1 g/l glucose) with 10% FBS and 1% anti-anti in a T25 flask. The cells were passaged into a T75 flask after they had grown and clustered, as shown...
26 February 2024 2,336 2 View
I have recently begun working with the HepG2 cell line to induce insulin resistance using a high-glucose environment. I acquired the cells from a -80°C storage where they had been since 2019, and...
10 February 2024 1,146 3 View
I am working a system of 40 atoms (Hafnium Selenide,monoclinic structure). When I try run the command "mpirun -np 8 VASP " , process terminates with this error. I have 8 GB RAM and I tried all...
12 January 2024 1,324 3 View
My research found that the application of bioencapsulated Bacillus along with transplanting is better than application before transplanting, assuming that applying along with transplanting allows...
24 November 2023 3,933 2 View
The aim of my project is to test the efficiency, advantages and disadvantages of 3-4 algorithms selected in two environments by using them on an autonomous delivery robot. These environments; The...
26 October 2023 3,752 4 View
I am spinning a solution for over 4 hours. the nanofibers I am collected are appearing textured and there’s some microdripping. At the same time, there’s smooth surface right next to it. What...
19 October 2023 9,146 4 View
During writing a review, usually published articles are collected from the popular data source like PubMed, google scholar, Scopus etc. My questions are 1. how we can confirm that all the...
19 October 2023 8,626 5 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I didn't find any data source for continuous time series since 1950. NHB Residex and RBI give data from 2013 and 2010 onwards. Please guide. Thanks !!!
27 July 2024 6,271 5 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
I am currently writing a systematic review
15 July 2024 9,176 1 View
Let me say I have a solar plant in operation and I want to build up a wind power plant, and later make a hybrid so that I could power the electrolyser as load for green hydrogen production.
14 July 2024 9,547 4 View
