What are the factors aaffecting the anthrone determination?

Maribel Ovando-Martínez @Maribel-Ovando-Martinez

21 May 2025 0 2K Report

I am using the anthrone assay in samples with ethanol to determine total carbohydrates. At the same time, I'm using reduced sugars by the DNS assay. However, when I compared the results, I saw that reduced sugars were higher than total carbohydrates. Do you think the ethanol percentage (40 and 80%) is causing this trend?

Badges
Science topic

Similar topics
Geoscience
Asia
India

More Maribel Ovando-Martínez's questions See All

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

Can I use DNA a ladder for RNA samples in agarose electrophoresis? Or It is preferable to use cDNA amplified from RT-qPCR for the same samples?

I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...

09 July 2024 4,857 2 View

Would the concentration of CFSE+ particles in EV samples be a more accurate way of measuring EV concentration?

Today, the most commonly used techniques for assessing the concentration of EV samples are NTA and the determination of total protein concentration. However, the concentration obtained by NTA...

16 May 2024 6,910 1 View

Agricuture, Innovation and Sustainability, Hot to encourage adoption and difussion in Spain ?

Which is the actual Status of Precission farming and regenerative farming in Castilla y Leon, Spain, which are the drivers that encourage adoption, and the potentil incentives, may catbon...

12 April 2024 7,901 0 View

How to determine variance of measurements between tests for each sample individually?

Hi, I am hoping to get some help on what type of statistical test to run to validate my data. I have run 2 ELISAs with the same samples for each test. I did perform a Mann-Whitney U-test to...

25 March 2024 7,474 4 View

?que imagen tiene el tulipán de un árbol de filogenético?

necesito una imagen de un filograma o árbol filogenético de un tulipán

23 February 2024 7,673 0 View

?imagen de un árbol filogenético de la flor la risa canina?

Necesito una imagen de un árbol filogenético de la flor de la rosa canina

22 February 2024 3,477 0 View

Is thiere a research similar to this?

Development of an Environmental Metal-Saltwater Battery for Alternative Power Generation

11 February 2024 4,227 0 View

Can intracellular proteins fused to GFP be detected by flow cytometry?

If cells were transfected with a plasmid containing a gene of interesed (the resulting protein is intracellular) fused to GFP, would the GFP signal be detected by flow cytometry? Or can we only...

19 January 2024 888 1 View

What is the gold standard ratio for phenotype:healthy control for EHR phenotyping?

Hello! I am working on a proposal for creating an electronic health record phenotyping classification algorithm (mental health focus). I am having a hard time finding solid guidance re:cohort...

17 January 2024 8,856 2 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

What is the Principle of Cysteine-carbazole-sulfuric acid reaction with glucose?

I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...

23 July 2024 3,891 0 View

Why does total carboydrate content decrease when increasing the vacuum degree?

I have conducted a survey on the "effect of vacuum drying conditions on carbohydrate content of angelica.S". The result show that, when increasing the vacuum degree, the carbohydrate content first...

13 July 2024 592 1 View

Do you know about fully Ethanol toyata Inova car?

https://youtu.be/5MckkqF9XWU?si=MvBsElJZg8_At8qP

09 July 2024 6,038 5 View

Is gasohol or Ethanol petrol mixture safe , ch?

What is safe gasoline and Ethanol safe or Ethanol petrol mixture safe for car fuelin.

03 July 2024 2,939 3 View

Why does a line appear in the middle of the carbohydrates TLC plate?

The line appears always in the penultimate run. We try to decrease the volume of the solvent system, wich is composed of butanol:acetic acid:water (2:1:1) (76 mL) but the line remains. Below I...

24 June 2024 9,829 3 View

Can I dissolve DPPH in THF for antioxidant activity Assay ?

My compounds are soluble In THF, so I woould like to know If for antioxidant activity I can prepare DPPH in THF. Before, I did a test in wich I had DPPH in ethanol, but when I Add my sample in...

17 June 2024 4,183 3 View

3T3-F442A cells not adhering to coverslip?

Hi, I am attempting to culture 3T3-F442A cells and carry out IF staining. I'm testing a variety of different compounds on the cells (e.g. lopinavir, clozapine, olanzapine etc.) Up until recently,...

16 June 2024 2,754 0 View

Why is Ethanol not precipitating our BSA?

We are trying to use 100% ethanol as a precipitant for Bovine Serum Albumin. The BSA is in a 0.5% concentration and we are using various ratios of ethanol to attempt to precipitate it, such as 1:2...

04 June 2024 8,024 1 View