Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

In the paper mentioned can you help me to find the finite difference of equation no 19.?

In the paper "Magnetometer-Only Attitude Determination Using Novel Two-Step Kalman Filter Approach" in equation 19 do I need to complete quaternion multiplication first and then do simple...

15 June 2020 7,522 4 View

How can I calculate excited state raman intensities in Gaussian09 ?

I am doing calculation to see excited state and ground state raman intensities. I have successfully done it for ground state using dft and after finding absorption wavelength , have optimized the...

08 November 2019 7,920 3 View

How to align reverse and forward DNA sequences in chromus software ??

Need detailed procedure to align DNA seq. before performing BLAST.

09 January 2019 7,584 3 View

For detecting single nucleotide mutation, do we use traditional PCR or qPCR?

I have got a deletion of single base pair thymine at 3744 position in BRCA1 gene, which PCR technique should I use to detect our gene mutation ?

05 March 2018 3,492 3 View

How to calculate grafting % for two polymers?

Hello, I am performing grafting of two polymers using microwave assisted method. I want to know that what is the correct method to assess and calculate grafting % among the two polymers. Is...

04 February 2018 6,874 3 View

How can you ensure that the drug is completely separated from liposomes?

After doing either sephadex column or ultrafiltration. How do you ensure that entire unentrapped drug is removed from liposomes? Will there be any extra peak in DLS?

10 January 2018 1,293 6 View

Can you suggest best cost effective CO2 incubator selling company for animal cell culture?

Hi I am looking for best cost effective CO2 incubator for animal cell culture facility. There are lot of models by various companies with different features. As such, so many options and hence, a...

20 August 2017 1,957 7 View

I want to study rearrangement of ROS 1 and RET genes in FFPE sample?

24 December 2016 4,778 1 View

What is the actual molecular weight of CD63 for exosome identification?

Hii, I always get variable result for CD63 immunoblot from exosomal protein as well as cellular protein of RAW cell line macrophages.I found some paper where it mentioned that CD63 has alternative...

29 November 2016 4,536 0 View

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02 March 2021 3,060 3 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View