In my previous understanding, E. gracilis accumulates paramylon when grown in high-carbon medium, especially if grown in the dark. I have reproduced this in the past using KH medium. However, I surprisingly found yesterday that E. gracilis grown in AF6 medium with 12:12 ligh:dark cycles for almost a month showed quite high paramylon content. Can anybody point me towards literature to explain this? Is there any understanding of the dynamics of this accumulation of paramylon?