I need to crosslink my cells for my experiment and at the end, I need to reverse crosslink and extract the proteins. Can anyone tell me what protocols are there for reverse crosslinking after which I can extract the proteins?
It depends on a crosslinker which has been used. If you used formaldehyde high concentration of salt and higher temperature would help. For disulfide-cleavable crosslinkers you can go with TCEP or DTT just to reduce a disulfinde bonds.