Consensus sequence from ClustalX?

I am doing multiple sequence alignment to design primer for my gene. Can anyone tell me how to select the consensus sequence after alignment. I am using ClustalX for the alignment.

04 February 2021 8,872 2 View

How to vary the implantation dose in simulation using SRIM software?

Hi, I am wondering if someone can direct me to an ion implantation forum My specific query is: I am learning the SRIM software for simulating ion implantation process. I am however, not finding...

05 May 2020 1,301 2 View

Colorimetric Assay for Total Cholesterol estimation without reagent kit?

I need to calculate the concentration of Total cholesterol in rat tissue samples. can anyone provide me a colorimetric assay without using any kits

19 February 2020 3,273 3 View

How to get DDSDDE for strain softening material or damage elastic material?

I am trying to implement a continuum damage mechanics based constitutive model in Abaqus using the user-defined subroutine UMAT. In that context, I am having hard time finding any available...

22 October 2019 9,426 3 View

T47D and HCC-1954 siRNA transduction using RNAiMAX(invitrogen) protocol?

Hello! I've been trying siRNA knockdown using RNAiMAX (invtrogen) but I'm not getting any significant knockdown in the T47D and HCC-1954. I'm using forward transfection. The protocol I uses is: 1....

15 March 2018 5,626 2 View

Why gold sputtering is needed before doing SEM for some polymer membrane samples?

I was making a polymer membrane for hydrogen fuel cell. in some of the papers i found people have done gold sputtering before SEM ?

05 March 2018 1,746 2 View

Can anyone provide recommendation for quantifying anthocyanins from plants using HPLC?

Looking for protocols to quantify anthocyanins using HPLC ( what standards to use and what column??)

21 February 2018 1,908 4 View

How to extract RNA from pseudobulb of medicinal orchids?

I am trying to extract RNA from the pseudo bulb part of a medicinal terrestrial orchid. although I got high quality RNA from leaf but while trying so from the pseudoibulb or rhizome I am facing...

27 December 2017 2,716 1 View

Can someone let me know if SiC epilayers on SiC single crystals with selective doping are available commercially?

I know that doped SiC epitaxial layers on SiC single crystals are available commercially. Is it also possible to buy SiC epilayers with some regions with n-type and some regions with p-type...

26 April 2017 9,583 4 View

How to get successful hairy root culture of carrot?

I want to do Root Organ Culture of carrot. I have tried with agrobacterium rhizogenes mtcc 532. i have tried with 8hrs, 12 hrs culture, 24 hrs, 48 hours culture.. i have collected cells by...

12 March 2017 4,581 5 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Could the "incoming radiation (net radiation)" values be negative?

Could the "incoming solar radiation" values be negative? What is the difference between "global solar radiation" and "incoming solar radiation"? Which one might be measured negative values?

21 February 2021 7,726 9 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Can I follow Researchers from Teaching and testing areas?

I want Researchers from Educational Measurement and Evaluation, relating to teaching, learning , academic performance and test validation

18 February 2021 4,757 13 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View