Thank you so much Kunal Dutta for attention and assistance

hi see this attach about criteria for ARMS-PCR primers

Thank you Noor . in the present time I am use Pimer 1 for design ARMS-PCR primer well but I want more efficient program. thank you again

Try this

PRIMER1: primer design for tetra-primer ARMS-PCR

primer1.soton.ac.uk/primer1.html