What are the controls for immunoprecipitation that will be excised and sent for MS for protein detection?

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Respected academician, I did staining of dormant and non-dormant seeds. Dont know how to explain these pics? please help ?

Peroxidase activity 📷📷 Dormant seed Non dormant seed

17 April 2024 7,384 2 View

Where do I obtain Chenopodium quinoa Willd. (quinoa) seeds (SAMN04338310; QQ74) that were originally used for genome sequencing?

The Chenopodium quinoa Willd. (quinoa) seeds with accession PI614886 (Biosample accession code SAMN04338310; also known as NSL 106399 and QQ74) is what I require and was also used for genome...

10 December 2023 5,680 2 View

What is the difference between sample preparation and sample cleanup?

Is sample cleanup not a part of sample preparation. Are there any examples sample cleanup that are not sample preparation in analytical chemistry?

19 October 2023 5,809 8 View

How can we treat the data if two latent variables have perfect correlation while using structural equation modeling?

05 June 2023 7,962 2 View

How does endoparasite develop inside the body ? Are they internally made or go inside the body from external environment through the means of feeds?

If we create an artificial environment to an animal, so that the feeds/grasses/silage/forage, concentrate, and water whatever we provide are totally parasites and their eggs or larvae free, then...

09 February 2023 4,633 3 View

I have a DEM with an ellipsoidal height value. How can I convert that ellipsoidal height into orthometric height?

Query on conversion tool.

14 February 2022 9,245 3 View

What is the best method to get historical satellite imagery except google earth and earthexplorer?

I am looking for satellite imagery of last 10 years so that i can find out change detection in such place. The possible solution to download the historical solution is by using google earth pro...

14 August 2021 5,775 11 View

About non linear analysis?

hello everyone. i have multiple continuous dependent variables rated on scale along with categorical independent variables such as sex, study etc. while checking for linearity i realised that my...

30 July 2021 9,085 7 View

Can someone help me with result section(apa) for my thesis?

hi i have to write my result section for my thesis but I keep on getting stuck because of formatting and issues. for analysis i have have conducted multivariate glm with sex,studyfaculty as...

10 July 2021 1,094 8 View

What could be the effects of rapid maturity of chicken (i.e. from 60 days to 35-40 days) on chicken itself and its consumer?

Just curious to know if anyone would take this as a reasonable question and would explain their views on it.

08 July 2021 2,636 9 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Can I change concentration and brand of antibodies mid-experiment?

I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....

29 July 2024 5,961 2 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

What does negative readings in spectrophotometer denote. Was testing TSS content of bacterial cultures?

I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...

22 July 2024 9,576 4 View

Promotor observation in region annotation of RNA RIP-seq?

Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...

18 July 2024 1,579 2 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

How to detect phosphorylation on Western Blots?

Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?

15 July 2024 2,055 3 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Is perfusion required to stain brain tissue with every method?

I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...

14 July 2024 177 4 View