SOP for removing multilayer graphene from one side of the Ni foil and etching the Ni foil with FeCl3 solution?

I have CVD grown Graphene on two sides of the Ni foil. I have tried O2 plasma (Ar 15 sccm & O2 5 sccm) with 150 W power for about 5-10 mins (multiple trials) to remove graphene from one side...

27 December 2018 3,120 1 View

What may be the contaminant observed in animal cell culture ?

The following image uploaded is from our Sub-ventricular Zone (SVZ) derived neurosphere culture (mice), Primary culture - Passage 2 - Day 2. Thread like structures were observed at 20X and 40X....

27 June 2018 3,269 10 View

Does Glutamine free medium contain Glutamate?

I want to use glutamate free medium for my experiment but I could not find it. But the glutamine free medium is commercially available and I want to ask whether glutamine free medium contains...

12 June 2018 8,237 3 View

How to preprocess multispectral image with low pixel values in shaded areas?

I have WV 3 image with very low pixel values in the shaded areas. Image covers a very large area and it is very clear. I am trying to do radiometric and atmospheric correction (ENVI FLAASH and...

28 December 2017 5,631 3 View

Identification of Azolla?

I need to identify whether or not the attached figure is Azolla ( Water fern)?

17 April 2017 2,830 11 View

While working on rhizodeposits assisted MFC, Is it common to get negative voltage near end of life cycle of plants due to replenishment of exudate?

17 October 2016 3,719 3 View

Where can I order aminomethylenediphosphonate (AMDP)?

AMDP is a specific inhibitor of proton pyrophosphatase. No vendor seems to be selling this chemical. Any help will be highly appreciated.

08 October 2015 9,603 3 View

What is most efficient DNA mismatch cleavage enzyme?

I am trying to cleave dsDNA with a single mismatch.

21 January 2015 8,075 3 View

What is the mechanistic role of DTT in ligation?

I could not find how DTT helps in ligation. I am looking an exact role of this compound.

11 December 2014 5,573 5 View

Is there any database where I can find all proteins expressed in the liver or any other organs?

I need to find the set of proteins expressed exclusively in liver. Please suggest any database or website.

03 October 2014 9,413 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

What is the best way to manage Western blots?

Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...

23 February 2021 8,609 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...

22 February 2021 1,777 3 View