Hi all.
After growing and inducting the E.coli culture, I extracted the cells by centrifugation, but there are some black spots in the brown pellet.
I was wondering if you observe these black spots too?. I read that It can be dead cells or inclusion bodies..
PS: I have grown these cells before but never observed them until the last two times. In practice, the protocols are identical as in past.
Hi, when my culture is cloudy after the induction and I harvest cells I usually find a little black spot into my light brown (when I perform culture in LB medium) E.coli pellet. Instead, when my culture is slightly cloudy I recover low quantity of dark brown pellet and it means that cells are dead.
I am agree with Viliano Vivenzio . After Induction, always light browh spots are seen and they belong to dead cells . They are very normal to be seen .
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