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Questions related from Deepak Deepak
Hello all, How to have multiple papers under one reference in any of the reference management tool (Mendeley/Zotero/Endnote)? For example ... is widely used in modern chemistry.[5] ... [5] a) A....
02 October 2023 7,332 1 View
Hi all. After growing and inducting the E.coli culture, I extracted the cells by centrifugation, but there are some black spots in the brown pellet. I was wondering if you observe these black...
13 May 2022 9,271 2 View
Dear all, I converted a PDB file into Mol2 file using Openbabel (H added) and then fixed the bond order number using this command: perl sort_mol2_bonds.pl jz4.mol2 jz4_fix.mol2 When I try to...
23 December 2020 536 4 View
Hi all, I am using NAP-5 disposable columns from GE for removing DTT from my protein. Can I use the same column again for the purpose of removing DTT. Can you please mention the protocol for...
11 December 2020 3,528 5 View
I want to reduce the disulfide bridge in my 7kDa protein. My protein has only 1 cysteine at the surface and thus makes a homodimer using a disulfide bridge. I want to reduce the disulfide bridge...
25 November 2020 3,396 5 View
Hi all, I have a purified protein (57kDa) which tends to precipitate at 4 deg C after a week (conc. 9mg/ml). Can you please share the protocol to flash freeze protein in Liquid nitrogen and...
17 September 2020 1,623 5 View
Dear all, I am making a 10mM KHPO4 buffer (pH 7) for protein purification. I need to add 350mM Imidazole to it for the purpose of protein elution, but after the addition of Imidazole pH jumps...
24 April 2020 1,212 4 View
Dear all, I was wondering if there is a database where I can find a list of proteins which are easy to express, purify and crystallize. Please suggest with your experience proteins which fits...
25 July 2019 5,866 0 View
Hi all, I have a protein to which a DNA is bound (pdb file). I want to align/superpose an organic molecule on to the top of DNA in that protein. Please let me know which software can do this and...
05 April 2019 2,438 3 View
I want to extract the DNA from these above-mentioned cell lines by using the organic solvent method. I don't want to use any isolation kit from any brand.
01 January 1970 1,997 6 View