Hi,
I am interested in learning more about technologies that focus on gene expression. Could you please recommend the best references to gain a solid understanding of the techniques related to gene expression?
Thank you.
Become very familiar with MIQE guidelines - https://www.gene-quantification.de/miqe.html
This book in Amazon Kindle ebook is also great - https://www.amazon.com/Introduction-PCR-Definitive-qPCR-Book-ebook/dp/B00DFNRU02/ref=sr_1_3?crid=3NNU6LKW515CC&dib=eyJ2IjoiMSJ9.-9rp63nZ_vEwGdxzRDRX1DPprCQefeLIwVKd0pNBNalo3nwgYHuGu5euuqpTMSIz1WZo-t5HxdGueu5LClt9iwZRLeqCxgwbDeLXQmKToFEomh5xt_KiClqBiPQorCOyWXgyt5sSwQQV2R3KqxcT0A.DfAOyxXUmT8-Motb3ipZuzDbu0LQ71isnAWUZZz-v-E&dib_tag=se&keywords=real+time+PCR&qid=1719779336&s=digital-text&sprefix=real+time+pcr%2Cdigital-text%2C194&sr=1-3
Book: PCR Protocols
Author(s): John M. S. Bartlett, David Stirling (auth.), John M. S. Bartlett, David Stirling (eds.)
Series: Methods in Molecular Biology 226
Publisher: Humana Press, Year: 2003
ISBN: 9780896036277,9781592593842,0896036278
I am not an expert in this field, but I am very interested and have researched to find an answer. Could you please review the response below to see if it is correct?
To gain a comprehensive understanding of gene expression techniques, I recommend exploring various platforms and methodologies. Start with "Gene Expression Commons," which provides an open platform for absolute gene expression profiling using extensive microarray data [1]. Next, delve into the application of CRISPR-Cas systems for fine-tuned gene expression control in bacteria [2]. For insights into next-generation sequencing (NGS) technologies in plants, refer to the review discussing NGS advantages and bioinformatics challenges [3]. Additionally, examine single-cell gene expression profiling for high-resolution data on cellular heterogeneity [4][5]. These references collectively offer a robust foundation in gene expression technologies.
Reference
[1] Seita, J., Sahoo, D., Rossi, D. J., Bhattacharya, D., Serwold, T., Inlay, M., Ehrlich, L. R., Fathman, J., Dill, D., & Weissman, I. (2012). Gene Expression Commons: An Open Platform for Absolute Gene Expression Profiling. PLoS ONE, 7.
[2] Vigouroux, A., & Bikard, D. (2020). CRISPR Tools To Control Gene Expression in Bacteria. Microbiology and Molecular Biology Reviews, 84.
[3] Jain, M. (2012). Next-generation sequencing technologies for gene expression profiling in plants.. Briefings in functional genomics, 11 1, 63-70 .
[4] Levsky, J., Shenoy, S., Pezo, R., & Singer, R. (2002). Single-Cell Gene Expression Profiling. Science, 297, 836 - 840.
[5] Ståhlberg, A., Kubista, M., & Åman, P. (2011). Single-cell gene-expression profiling and its potential diagnostic applications. Expert Review of Molecular Diagnostics, 11, 735 - 740.
I received some assistance from tlooto.com for this response.
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